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</table>Adminhttps://unilogia.su/index.php?title=Enhancer_trap&diff=893&oldid=prevru>Kalysc: /* See also */ added P element2020-04-28T18:34:45Z<p><span class="autocomment">See also: </span> added P element</p>
<p><b>Новая страница</b></p><div>An '''enhancer trap''' is a method in [[molecular biology]]. The enhancer trap construct contains a [[transposable element]] and a [[reporter gene]]. The first is necessary for (random) insertion in the genome, the latter is necessary for identification of the spatial regulation by the enhancer. On top of this, the construct usually includes a [[genetic marker]], e.g., the [[White (mutation)|white gene]] producing red-colored eyes in ''[[Drosophila]]'', or ampicillin resistance in ''[[Escherichia coli|E. coli]]''.<br />
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The most common and basic enhancer traps are: P<nowiki>[</nowiki>[[lacZ]]<nowiki>]</nowiki> from the [[bacterium]] ''[[Escherichia coli|E. coli]]'' and P<nowiki>[</nowiki>[[GAL4/UAS system|GAL4]]<nowiki>]</nowiki> from [[yeast]]. There exists a large number of fly stocks containing GAL4 insertions and an equally large number of fly stocks containing an UAS DNA sequence followed by a gene of interest, which permits the expression of a large number of genes with different GAL4 "drivers". Rather than generating transgenic flies with the enhancer linked directly to the gene of interest (which takes about a year when starting without the appropriate DNA construct), one transgenic fly is simply mated (crossed) with another transgenic fly.<ref>[[Andrea Brand]] and [[Norbert Perrimon]] (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development, 118, pp 401-415.</ref><ref>D. J. Finnegan (1992). Transposable elements. Current Opinion in Genetics and Development, 2, pp 861-867.</ref><ref>J. A. Fischer, E. Giniger, T. Maniatis and M. Ptashne (1988). GAL4 activates transcription in Drosophila. Nature, 332, pp 853-856.</ref><br />
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==See also==<br />
*[[Gene trapping]]<br />
*[[P_element|''P'' element]]<br />
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==References==<br />
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[[Category:Genetics]]</div>ru>Kalysc