https://unilogia.su/index.php?action=history&feed=atom&title=Recognition_sequenceRecognition sequence - История изменений2026-04-09T01:42:04ZИстория изменений этой страницы в викиMediaWiki 1.44.2https://unilogia.su/index.php?title=Recognition_sequence&diff=848&oldid=prevAdmin: 1 версия импортирована2025-11-13T17:59:58Z<p>1 версия импортирована</p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="ru">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Предыдущая версия</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Версия от 17:59, 13 ноября 2025</td>
</tr><tr><td colspan="4" class="diff-notice" lang="ru"><div class="mw-diff-empty">(нет различий)</div>
</td></tr>
<!-- diff cache key mediawiki:diff:1.41:old-847:rev-848 -->
</table>Adminhttps://unilogia.su/index.php?title=Recognition_sequence&diff=847&oldid=prevru>Aadirulez8: v2.05 - Autofix / Fix errors for CW project (Link equal to linktext)2024-12-20T16:26:33Z<p>v2.05 - Autofix / Fix errors for <a href="/index.php?title=WP:WCW&action=edit&redlink=1" class="new" title="WP:WCW (страница не существует)">CW project</a> (Link equal to linktext)</p>
<p><b>Новая страница</b></p><div>{{Short description|DNA sequence (or subset thereof), to which the domain is specific}}<br />
A '''recognition sequence''' is a [[DNA sequence]] to which a [[structural motif]] of a [[DNA-binding domain]] exhibits [[Chemical specificity|binding specificity]]. Recognition sequences are [[Palindromic sequence|palindromes]].<ref>{{cite journal|title=One recognition sequence, seven restriction enzymes, five reaction mechanisms| pmc=443551 | pmid=15226412 | doi=10.1093/nar/gkh685| volume=32| year=2004| journal=Nucleic Acids Res| pages=3469–79 | last1 = Gowers | first1 = DM | last2 = Bellamy | first2 = SR | last3 = Halford | first3 = SE| issue=11 }}</ref><br />
<br />
The [[transcription factor]] [[Transcription factor Sp1|Sp1]] for example, binds the sequences 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3', where (G/T) indicates that the domain will bind a [[guanine]] or [[thymine]] at this position.<br />
<br />
The [[restriction endonuclease]] [[PstI]] recognizes, binds, and cleaves the sequence 5'-CTGCAG-3'.<br />
<br />
A '''recognition sequence''' is different from a '''[[recognition site]]'''. A given recognition sequence can occur one or more times, or not at all, on a specific DNA fragment. A '''recognition site''' is specified by the position of the site. For example, there are two PstI recognition sites in the following DNA sequence fragment, starting at base 9 and 31 respectively. A recognition sequence is a specific sequence, usually very short (less than 10 bases). Depending on the degree of specificity of the protein, a DNA-binding protein can bind to more than one specific sequence. For PstI, which has a single sequence specificity, it is 5'-CTGCAG-3'. It is always the same whether at the first recognition site or the second in the following example sequence. For Sp1, which has multiple (16) sequence specificity as shown above, the two recognition sites in the following example sequence fragment are at 18 and 32, and their respective recognition sequences are 5'-GGGGCGGAGC-3' and 5'-TGGGCGGAAC-3'.<br />
<br />
5'-AACGTTAG'''CTGCAG'''TC''GGGGCGGAGC''TAGG'''CTGCAG'''GAAT''TGGGCGGAAC''CT-3'<br />
<br />
==See also==<br />
{{Wikidata property|P4863}}<br />
*[[DNA-binding domain]]<br />
*[[Transcription factor#Classes]], for more examples<br />
<br />
==References==<br />
{{reflist}}<br />
<br />
{{DEFAULTSORT:Recognition Sequence}}<br />
[[Category:Genetics]]<br />
[[Category:Genome editing]]<br />
[[Category:Protein structural motifs]]<br />
<br />
<br />
{{Genetics-stub}}</div>ru>Aadirulez8