https://unilogia.su/index.php?action=history&feed=atom&title=Tre_recombinase 2026-04-09T01:43:44Z История изменений этой страницы в вики MediaWiki 1.44.2 https://unilogia.su/index.php?title=Tre_recombinase&diff=908&oldid=prev 2025-11-13T18:00:22Z

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<p><span class="autocomment">top: </span> <a href="/index.php?title=%D0%A3%D1%87%D0%B0%D1%81%D1%82%D0%BD%D0%B8%D0%BA:Monkbot/task_21:_Replace_page(s)_with_article-number&amp;action=edit&amp;redlink=1" class="new" title="Участник:Monkbot/task 21: Replace page(s) with article-number (страница не существует)">Monkbot/task 21: Replace page(s) with article-number</a>;</p> <p><b>Новая страница</b></p><div>&#039;&#039;&#039;Tre recombinase&#039;&#039;&#039; is an experimental [[enzyme]] that in lab tests has removed [[DNA]] inserted by [[HIV]] from infected cells.&lt;ref name=&quot;pmid17600219&quot;&gt;{{cite journal |first1=Indrani |last1=Sarkar |first2=Ilona |last2=Hauber |first3=Joachim |last3=Hauber |first4=Frank |last4=Buchholz |year=2007 |title=HIV-1 proviral DNA excision using an evolved recombinase |journal=[[Science (journal)|Science]] |volume=316 |issue=5833 |pages=1912–15 |pmid=17600219 |doi=10.1126/science.1141453 |bibcode=2007Sci...316.1912S |s2cid=2437602 }}&lt;/ref&gt; Through [[directed evolution|selective mutation]], [[Cre recombinase]] which recognizes loxP sites are modified to identify HIV [[long terminal repeats]] (loxLTR) instead. As a result, instead of performing [[Cre-Lox recombination]], the new enzyme performs recombination at HIV [[provirus]] sites.&lt;ref&gt;{{cite journal |title=Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice |first1=Ilona |last1=Hauber |first2=Helga |last2=Hofmann-Sieber |first3=Jan |last3=Chemnitz |display-authors=3 |first4=Danilo |last4=Dubrau |journal=PLOS Pathogens |volume = 9|issue = 9|article-number = e1003587|date=September 26, 2013 |doi=10.1371/journal.ppat.1003587 |pmid = 24086129|pmc=3784474 |doi-access=free }}&lt;/ref&gt;<br /> <br /> The structure of Tre in complex with loxLTR has been resolved ({{PDB|5U91}}), allowing for analyzing the roles of individual mutations.&lt;ref&gt;{{cite journal |last1=Meinke |first1=G |last2=Karpinski |first2=J |last3=Buchholz |first3=F |last4=Bohm |first4=A |title=Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA. |journal=Nucleic Acids Research |date=19 September 2017 |volume=45 |issue=16 |pages=9726–9740 |doi=10.1093/nar/gkx603 |pmid=28934476 |pmc=5766204 |doi-access=free}}&lt;/ref&gt;<br /> <br /> ==References==<br /> {{Reflist}}<br /> <br /> ==External links==<br /> * {{cite news |url=http://www.sciam.com/article.cfm?articleID=737AB56E-E7F2-99DF-382B756D1860EACA&amp;chanID=sa003 |title=Designer enzyme cuts HIV out of infected cells |work=[[Scientific American]] |date=June 28, 2007 |first=JR |last=Minkel }}<br /> <br /> {{Genetics-stub}}<br /> {{Antiretroviral drug}}<br /> <br /> [[Category:Genetics]]<br /> [[Category:Molecular biology]]</div>

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